GC-LC MS/Gas and Liquid Chromatography-Mass Spectrometry

Mass spectrometry (MS) is a powerful qualitative and quantitative analytical technique based on the mass-to-charge ratio (m/z) of ions generated from a sample. Mass spectrometers typically consist of an ionization source, an analyzer, and a detector. Mass analyzers can also be combined to form tandem mass spectrometers (MS/MS) enhancing more the capabilities of the technique.

The coupling of MS with the powerful separation capabilities of gas chromatography (GC-MS) and liquid chromatography (LC-MS) led to their widespread applications in various scientific fields i.e. chemistry, environment, and medicine, among others. Electron impact ionization (EI) is by far the most used ionization method in GC-MS, in which energetic electrons interact with gas-phase molecules to produce ions, M+. This technique is particularly useful for the detection of volatile and thermostable compounds.

In LC-MS, electrospray (ESI) and Atmospheric pressure chemical ionization (APCI) are commonly used ionization methods. In ESI, charged ions of molecules are produced using an electrospray, whereas APCI makes use of ion-molecule reactions that take place at atmospheric pressure. These techniques are used in general for the analysis of polar, non-volatile molecules.

The facility offers GC-MS and LC-MS for both qualitative and quantitative analysis.

Tool

Liquid Chromatography-Mass Spectrometry (GL-MS) system (Thermo Electron Corporation)

Applications

Quantitation and quantification of organic molecules

Mass Spectrometer

Finnigan TSQ Quantum Discovery Max triple-stage quadrupole mass spectrometer

Ionization Source

Atmospheric pressure chemical ionization (APCI)
Electrospray ionization (ESI)

LC Pump

Finnigan Surveyor LC pump Plus

Autosampler

Finnigan Surveyor Autosampler Plus

Chromatographic Column

Atlantis T3, 2.1 mm × 100 mm, 3 μm (Waters)
Atlantis dC18, 2.1 mm × 100 mm, 3 μm (Waters)
Zorbax eclipse XDB-C18, 4.6 mm × 50 mm, 1.8 μm (Agilent)
Poroshell 120 EC-C18, 2.1 mm × 100 mm, 2.7 μm (Agilent)

Analysis Software

Xcalibur

Additional Information

Dr. Anastasia Hiskia, a.hiskia@inn.demokritos.gr

Tool

Gas Chromatography-Mass Spectrometry (GC-MS) system (Agilent)

Applications

Quantitation and quantification of organic molecules

Gas Chromatograph

Agilent 6890 Series gas chromatograph

Detector

Agilent 5973 Network mass selective detector

Autosampler

HP (Hewlett-Packard) 6890 Series injector

Chromatographic Column

Vocol fused silica capillary column, 30 m × 0.25 mm, 1.5 μm (Supelco)

Analysis Software

MSD ChemStation

Additional Information

Dr. Anastasia Hiskia, a.hiskia@inn.demokritos.gr

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