HR-LS-NMR / High-Resolution Liquid-Sate Nuclear Magnetic Resonance

High-Resolution Liquid-State NMR (HR-LS-NMR) spectroscopy is used for structure elucidation and characterization of molecules and materials in solution. The method is based on the interactions of nuclear spins of the atoms in the molecules with a powerful static magnetic field created by an energized superconducting coil immersed in liquid Helium. The NMR active isotopes of the nuclei (spinning positive charges) possess angular momentum and a magnetic dipole moment. Thus when placed inside the strong magnet assume discrete orientations (energy levels) with respect to the external field. When a pulse at the correct resonance frequency (energy) for a nucleus in the sample induces transitions among the energy levels, signals are detected at characteristic frequencies (chemical shifts). In liquids, the rapid tumbling of molecules averages anisotropic (direction-dependent) effects resulting in narrow spectral lines close to 1 Hz with clearly defined multiplets, revealing the fine detail of interactions among nuclei and electrons in the molecule that are translated to bond connectivity and spatial proximity of the atoms. NMR experiments using a variety of specifically designed pulse sequences can provide a wealth of information regarding the exact molecular structure of small, medium and very large molecules as well as 3D molecular structures of complicated systems.

Moreover, specific applications of interest for researchers as well as the industrial sector include: structure elucidation of specific additives or contaminants and pollutants; quantitative analysis (chemical compo­sition determination, formulation investigation, raw material fingerprinting, mixture analysis, sample purity determination); compound identification or presence confirmation in mixtures; identification and conformational analysis of chemicals, synthetic or natural ; characterization of soluble materials (e.g. polymers); monitoring of compound stability and investigation of chemical transformations with time or temperature.


500 MHz (11.7 Tesla) Bruker Avance NMR spectrometer
250 MHz (5.8 Tesla) Bruker Avance III NMR spectrometer


High Resolution Nuclear Magnetic Resonance (NMR) spectroscopy for liquid samples
Direct and indirect observation of the 1H, 13C, 15N, 31P, and 19F nuclei in liquid samples, as well as 2D 1H-1H and 1H-X (X = 13C, 15N, 31P) correlation experiments


500 MHz: BBI inverse broad band with z-gradients;
BBO direct broad band
250 MHz: Dual 1H/13C direct with z-gradients

Temperature Control

500 MHz: BVT3000
250 MHz: BVT3000
Both instruments: Low T accessory down to -100oC

Sample Size

Both instruments accept 5 mm tubes

Processing Software

500 MHz: Bruker Topspin 1.3
250 MHz: Bruker Topspin 2.1

Additional Information

Dr. Konstantina Yannakopoulou,

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