SLS – DLS / Static and Dynamic Light Scattering

Static and Dynamic Light Scattering (SLS / DLS). Light Scattering is a physical phenomenon caused by the interaction of light with matter.

Static Light Scattering is an optical technique that measures the intensity of the scattered light as a function of the scattering angle and provides information on the absolute value of the molecular weight of (macro)molecules, the radius of gyration, the form and the structure factor as well as the A2 coefficient.

Dynamic Light Scattering, also known as photon correlation spectroscopy is best known for its use in sizing covering nm to micrometer. DLS probes dynamics in transparent samples by correlating the scattered light fluctuation at a given angle. The measured intensity time autocorrelation functions provide characteristic decay and decay time of the fluctuation present in the sample with the length scale and time scale. Its best known application is to measure particles sizes (hydrodynamics radius) through the measurements of their diffusion coefficients in dilute solutions.

Size range: nm to μm.

For more complex materials, DLS will require careful analysis.

It is noted that all DLS setups can be utilized for SLS measurements as well.

Innovation-el offers a broad range of instruments covering different type of dynamics, of samples environment and of optical conditions.

Tool

Home-made DLS apparatus using fiber optic collection of scattered light
Near-infrared excitation source

Applications

Particle size determination
Dynamics of inorganic supercooled liquids and melts
Thermoreversible phase changes

Temperature

Option for high temperature DLS measurements up to 300oC

Additional Information

Dr. Spyros N. Yannopoulos, sny@iceht.forth.gr

Tool

CGS-3 compact goniometer set up for simultaneous multi-angle SLS and DLS measurements.
Laser source: 22mW at λ=633 nm.
ALV 5000 correlator.
Avalance detector.
External bath for temperature control with circulator.

Applications

Molecular weight characterization, particle sizing, thermodynamic interactions in solutions, macromolecular self-assembly (structure and kinetics).
Macromolecular and protein interactions, macromolecular complexes, surfactant, liposomes, DNA and protein solutions characterization.
Thermoreversible structural and phase transitions.
Dynamics of complex fluids.

Temperature

-10 to 90oC, with external circulator.

Measurement And Analysis Software

ALV SLS and DLS analysis software

Sample Environment

Sample needs to be reasonably transparent.
Sample volume: microliter to milliliter.
Controlled temperature, pH and ionic strength sample conditions are available depending on the application.

Additional Information

Dr. Stergios Pispas, pispas@eie.gr

Tool

A wide variety of SLS and DLS setups are available:
Commercial setups for SLS and DLS
(a) LSI 3D-DLS
(b) ALV 5000 DLS
(c) BI-200SM DLS
Specific DLS set-up
2 axis internal reflection DLS,
High pressure DLS up to 15oC< T < 80oC, P up to 1500 bar
Multispeckle DLS for slow dynamics and nonergodic dynamics

Applications

Characterization, Particle sizing , Self assembly kinetics
Dynamics of complex fluids

Temperature

There is an extended temperature range that varies depending on the setup
Setup with goniometer (scattering angles: 150-11o); temperature: 15-65oC
Fixed angles; Temperature: RT-250oC

Measurement And Analysis Software

Analysis is required - it depends on the type of sample and the needed information

Sample Environment

Samples needs to be reasonably transparent
Sample volume : microliter to milliliter
Extended sample conditions are available, depending on the specific setup

Additional Information

Dr Benoit Loppinet, benoit@iesl.forth.gr
Dr. Kiriaki Chrissopoulou, kiki@iesl.forth.gr

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provided at Innovation-el by:
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IESL/FORTH Heraklion